BBMRI.at & COVID-19
COVID-19 caused by the SARS-CoV-2 corona virus represents a great challenge for our healthcare and economic systems. The situation and the working conditions are difficult in many places and direct patient care has the highest priority.
The Medical University BBMRI.at partners are doing a great job in patient diagnosis, care and treatment. In addition, there are also research activities.towards finding ways to deal with the COVID-19 disease. Biobanks can support researchers and provide key services to them, such as:
Efficient and high-quality storage of samples in clinical and research settings;
Samples from healthy individuals, to be used as control (collected 2-3 months before outbreak in each country);
Provide guidance and standards for targeted identification, collection and conservation of important samples.
Search the BBMRI-ERIC Directory for COVID-19 biobank sample collections ... open Directory
What BBMRI.at partners do
MED UNI GRAZ (with Institute of Pathology and Biobank Graz)
Austria's highest biosecurity laboratory and autopsy area (BSL-3)
Access to patient samples
Isolation of pathogens from autopsy cases (BSL-3)
SARS-CoV2 virus culture (BSL-3)
Sequence characterized SARS-CoV-2 variants isolated from Austrian patients
Isolated SARS-CoV-2 proteins from various virus variants
Isolated SARS-CoV-2 RNA quantified by digital PCR
Support in diagnostic assay development
COVID-19 convalescent cohort (saliva, serum, Li-Hep plasma, EDTA plasma, Na-Citrate plasma, EDTA buffy coat, nasopharyngeal swab; questionnaire on symptoms, 5 visits)
COVID-19 vaccination cohor (serum, PBMCs, saliva; 5 visits; immunosuppressed and healthy patients)
Prospective sample collection/study cohorts
Media reports about the High Security Lab at Med Uni Graz:
- ORF "Grazer Speziallabor im Einsatz gegen COVID-19" ... open report>>
- Med Uni Graz - GrazSpeziallabor für hochinfektiöses Material in Graz ... open report
MED UNI INNSBRUCK (with Biobank Innsbruck)
Samples from patients with COVID-19 collected in clinical setting from the Tyrol region (Austria COVID-19 hotspot), linked to clinical data.
MED UNI VIENNA (with MedUniWien Biobank)
Serum samples collected before the SARS-CoV-2 outbreak (for validation of serologic (IgG, IgM) tests, as those samples might be definitively free from antibodies against the virus);
Collections from patients with COVID-19 collected in clinical setting are currently in planning.
Samples from population-based cohorts collected after the SARS-CoV-2 outbreak (e.g. collected during prophylactic health examination programs, emergency department/primary care visits, etc.) that might help to estimate the actual seroprevalence of the virus in the different areas:
COVID-19 convalescent cohort
COVID-19 vaccination cohor
COVID-19 Collaboration Portal
Some guidelines for handling COVID-19 (SARS-Cov-2 containing) specimens:
Laboratory Biosafety Guidelines for Handling and Processing Specimens Associated with Coronavirus Disease 2019 (COVID-19)
Interim guideline (31 March 2020) ... open
FAQs about Laboratory Biosafety & SARS-CoV-2 from the Centers for Disease Control & Prevention (CDC)
View details on specimen handling https://www.cdc.gov/coronavirus/2019-ncov/lab/biosafety-faqs.html
WHO Laboratory biosafety guidance related to the novel coronavirus
Interim guidance (12 Feb 2020) .. open>>
Dealing with COVID-19 contaminated waste
from Austrian Federal Ministry for Climate Action, Environment, Energy, Mobility, Innovation and Technologyy ... open guideline (German)
Air Transport of COVID-19 Contaminated Samples
Novel Coronavirus (Covid-19)
Dangerous goods (including alcohol based sanitizers) guidance for Operators – 07 March 2020 ... open guideance
"... Based on the experience of shipping specimens of SARS, swine flu, MERS and other similar types of viruses, WHO and national health authorities such as the US Centers for Disease Control (CDC) are advising health authorities to ship specimens of Covid-19 as UN 3373, Biological substance, Category B. To ensure that the global response to suspected cases of Covid-19 is as rapid as possible, it is important that specimens can be transported to laboratories for analysis without delay. ..."
LOCAL REGULATIONS - Examples
- "Steirischer Seuchenplan">> … requires autopsies to be performed in BLS-3 laboratories
DEDICATED PUBLICATIONS - Examples
Overview on COVID-19 related publications at WHO website ... open >>
They provide results on SARS-CoV persistence on surfaces, during disinfection routine FFPE tissue processing. Many findings are from related SARS-CoV virus studies as publications on SARS-CoV-2 are limited.
Biospecimens containing SARS-Cov-2:
Heather A. Lankes, and Hala Makhlou (2020): Biospecimen Collection During the COVID-19 Pandemic Considerations for Biobanking,
Am J Clin Pathol 2020;XX:1–0, DOI: 10.1093/AJCP/AQAA171
- Current data suggest the earliest cases in the COVID-19 pandemic may have been as early as October 2019.
- Millions of biospecimens have been and will be collected from known and suspected cases, as well as asymptomatic and presymptomatic individuals for various purposes.
- Biosafety considerations intended to complement evolving CDC and WHO guidelines are presented.
PCR test on specimens from COVID-19 patients with fever, dry cough, and fatigue revealed that the virus was present in the following specimens;
Highest rates: in bronchoalveolar lavage fluid (14 positive results of 15 samples; 93%),
followed by sputum (72 of 104; 72%),
nasal swabs (5 of 8; 63%),
fibrobronchoscope brush biopsy (6 of 13; 46%),
pharyngeal swabs (126 of 398; 32%),
and feces (44 of 153; 29%).
Lower positive rates were found in and blood (3 of 307; 1%).
None in urine (0 of 72; 0% (
- Anthony F. Henwood (2020) Coronavirus disinfection in histopathology, Journal of Histotechnology, DOI: 10.1080/01478885.2020.1734718
This technical note presents disinfection procedures and histotechnology processes for corona virus containing specimens and using data obtained from similar coronaviruses, e.g. severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS).
- Darnell ME et al. (2004); Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV. J Virol Methods. 2004 Oct 1;121(1):85–91.
In older studies on SARS-CoV viruses, it was determined that formalin and glutaraldehyde inactivated SARS-CoV in a temperature- and time-dependent manner. Formalin fixation performed at 37°C or room temperature, "significantly decreased the infectivity of the virus on day 1, while glutaraldehyde inactivated SARS-CoV after incubations of 1–2 days.
- Duan et al. (2003); Stability of SARS coronavirus in human specimens and environment and its sensitivity to heating and UV irradiation. Biomed Environ Sci. 2003 Sep;16(3):246–255.
The authors found that several coronaviruses were made non-infectious after the following exposure times and temperatures: 90 min at 56°C, 60 min at 67°C, and 30 min at 75°C. Paraffin infiltration in most histopathology laboratories uses a temperature of 60–65°C for 2 h or more. It is, therefore, appropriate to consider that the formalin-fixed paraffin-embedded tissue block would have a low risk of coronavirus infectivity.
- Sheridan C. et al., Fast, portable tests come online to curb coronavirus pandemic. Nat Biotechnol. 2020 Mar 23. doi: 10.1038/d41587-020-00010-2
Second vaccination unnecessary for COVID-19 recovered patients
Study with Med Uni Wien Biobank involvement (ORF, August 2021)
Researchers from Graz rely on nasal spray with banana protein
TV report on RTL (17 Aug 2021)
Biosafety Requirements for Autopsies of Patients with COVID-19
Publication by BBMRI.at partner Med Uni Graz - Jan 2021
„Corona – auf der Suche nach der Wahrheit“
TV documentary with BBMRI.at partner Med Uni Graz (K. Zatloukal)
COVID-19 Genesene gesucht
Med Uni Graz, Biobank Graz - ab Juni 2020
Corona drug tests in Graz
at BSL-3 facility at BBMRI.at partner Med Uni Graz – April 2021
Tablet against COVID-19 transmission
research at universities in Graz
COVID-19: Tests and medicine candidates developed in Vienna, Austria
an overview – Dec 2020
Tyrolean anti-coronavirus nasal spray tested at BBMRI.at partner Med Uni Graz
ORF report, series ‘Konkret’ – 22 Nov 2020
Knowledge among pathogens
Ö1 radio interview with Prof. Zatloukal – 1 Sep 2020
SARS-CoV-2 test comparison
with involvement of MedUni Wien Biobank – Aug 2020
Guidelines & publications for SARS-Cov-2 containing biospecimens
a current summary - 1 April 2020
Austria's highest security BSL-3 lab at BBMRI.at partner Med Uni Graz
… is ready for research - 28 March 2020
COVID-19 research at BBMRI.at
.. report on SERVUS TV 3 Apr 2020
Biobanking in times of Covid-19
BBMRI-ERIC web discussion - 31 March 2020
BBMRI.at & COVID 19
What BBMRI.at partners do .... March 2020
COVID-19 research funding
... links to current fundings - March 2020