Delays during PBMC isolation have a moderate effect on yield, but severely compromise cell viability
One of BBMRI.at’s core competences is quality management and a particular focus is put on the pre-analytical phase, which can significantly alter an analytical outcome and lead to incorrect results.
Experts at Med Uni Wien Biobank therefore investigate a critical step during the isolation of peripheral blood mononuclear cells (PBMCs). PBMCs are widely used in clinical routine and research.
Their studies show that a 24h delayed processing of cells after Ficoll gradient centrifugation led to substantial decrease in cell viability with increased apoptosis.
Golke T, Mucher P, Schmidt P, Radakovics A, Repl M, Hofer P, Perkmann T, Fondi M, Schmetterer KG, Haslacher H. Delays during PBMC isolation have a moderate effect on yield, but severly compromise cell viability. Clin Chem Lab Med. 2022 Jan 27. doi: 10.1515/cclm-2022-0003. PMID: 35085430.
